The stepone software included with the steponeplus realtime pcr system runs on the windows xp operating system and provides instrument control, data collection, and data analysis capabilities analysis software is also compatible with windows 7. Does this machine analyse the qpcr data imported from abi 7300. Applied biosystems abi pcr stepone 48 wells realtime thermal. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Stepone and steponeplus realtime pcr system thermo fisher. Applied biosystems steponeplus for software version 2. The stepone 48 well and steponeplus 96 well realtime pcr systems are remarkably simple to use.
Software downloads stepone and steponeplus realtime pcr system note. These factorycalibrated onestep systems make it easy to step up to highperformance qpcr. This can be difficult because deleting this manually takes some knowhow related to windows program uninstallation. Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. Assays and reagents specifically tailored for many types of research can be ordered from applied biosystems through the stepone software. Applied biosystems abi stepone 48well realtime pcr system. Stepone and steponeplus realtime pcr system thermo. Applied biosystems stepone realtime pcr software help explains how to use the stepone software to. Watch the video, find your best fit, or request a quote. Set up, run, and analyze experiments using the stepone and steponeplus systems. Stepone software is a shareware software in the category desktop developed by applied biosystems. The software then creates colorcoded plate layout schemes for easy pipetting. Use only optical grade tubes, flat top caps or plates, see below for order information. G161544 applied biosystems 4376592 steponeplus realtime pcr.
How to use stepone plus qpcr machine by applied biosystems. The latest version of stepone software is currently unknown. A compact qpcr system, the applied biosystems stepone plus is available for quantitative realtime pcr analysis. Guide to running kasp genotyping reactions on the abi stepone. Field application specialist doug rains offers advices for fixing common software setup mistakes when performing real.
View online or download applied biosystems steponeplus installation, networking, and maintenance. The software program allows easy setup, monitoring during amplifications and data analysis. Monitor networked stepone and steponeplus instruments. You should try to export your data in a csv or txt file with the software of abi. The abi applied biosystems stepone real time pcr system is a 48well, lowthroughput realtime pcr instrument perfect for both first time and experienced users.
Software instrument software for the applied biosystems stepone and steponeplus realtime pcr systems runs on the windows xp operating system and provides instrument control, data collection, and data analysis. The stepone and steponeplus systems software contains. It provides the capability to monitor the progress of experiments, either remotely or on the machine, cyclebycycle. The abi stepone plus realtime pcr offers four color optical recording allowing for faster measurement. For example, data can be analyzed from multiple perspectives in the multiple plots view see figure 5. Applied biosystems stepone steponeplus realtime pcr systems. Amplify sample with the steponeplus real time system. The stepone realtime pcr system is a lowthroughput realtime pcr instrument perfect for both firsttime and experienced users.
Is there a maccompatible control software for the stepone rtpcr. The steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. The steponeplus realtime pcs stores the resulting data, which can then be transferred by email or usb. Steponeplus realtime pcr system from thermo fisher scientific. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. Jan 26, 2016 used abi stepone qpcr on sale at the lab world group.
Applied biosystems steponetm and steponeplustm realtime pcr systems site preparation guide 7 preface obtaining information from the help system the stepone software has a help system that d escribes how to use each feature of. Smart monitoring agent for the stepone and steponeplus systems. The abi steponeplus is an advanced real time pcr used for fast quantitative measurements. This userfriendly and intuitive software package includes the following features. The applied biosystems steponeplus realtime pcr system makes it simple and easy to get highquality realtime pcr results. How to label technical replicates in life technologies realtime pcr software ask. The applied biosystems steponeplus realtime pcr system is a robust, flexible instrument capable of performing a wide variety of genomic assays. This separately purchased software simplifies setup by maintaining assay specific settings and accepts pasted plate layout information directly from excel. Abi applied biosystems stepone real time pcr system. Steponeplus realtime pcr system from thermo fisher. Applied biosystems stepone qpcr comes with its own software for template input. The stepone plus was quoted to us at roughly 35k usd converted from our local supplier while the next higher abi models 7500 and quantstudio 5 and the qpcrs from other brands start at 55k usd. Entire experiments can be planned and executed in the stepone software. Fixing software setup mistakes in realtime pcr steponeplus.
Jun 26, 2012 fixing software setup mistakes in realtime pcr steponeplus ask taqman. Intuitive data analysis and instrument control software that performs for a wide. Were still waiting for the biorad cfx96 touch price. Stepone system reaction setup view software the stepone and steponeplus systems software contains unique features not available in other realtime pcr instruments. Instrument software for the applied biosystems stepone realtime pcr system runs on the windows xp operating system and provides instrument control, data collection, and data analysis. The steponeplus system brings advanced realtime pcr technology to a new level of accessibility.
Used applied biosystems stepone plus realtime pcr system. Realtime pcr abi prism 7500 psc 563, nsc 338 realtime pcr stepone psc 563 every time you use any of the qpcr instruments, you must sign the log book. After gathering some information about what the market of real. The applied biosystems stepone plus pcr machine has the ability to measure gene amplifications as theyre occurring in realtime, cycle by cycle, increasing precision measurements during the vital stage of the pcr process. This can be troublesome because performing this manually takes some skill related to pcs. Applied biosystems stepone plus shared resources fred. The stepone real time pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Searching for a new real time pcr machine which one. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast 40 min and standard 2 hr runs, and are configured for pcfree or networked operation. Applied biosystems stepone and steponeplus realtime pcr systems installation, networking, and maintenance guide explains how to install and maintain the stepone and steponeplus systems. Realtime pcr abi prism 7500 psc 563, nsc 338 realtime pcr. Applied biosystems stepone realtime pcr system, laptop. The best simple action to uninstall stepone software v2. The steponeplus realtime pcr system is a 96well realtime pcr.
And now these systems support high resolution melt hrm software. It was initially added to our database on 10222008. Stepone software runs on the following operating systems. The stepone realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software. Applied biosystems stepone and steponeplus realtime pcr systems. This document is intended as a guide to running kasptm genotyping reactions on the abi stepone and steponeplus instruments.
It can read most commercially available dyes such as fam, hex, rod, cybr, cy3, cy5 and more. Stepone system with the gene expression study package. For our lab we are considering the purchase of a new real time pcr machine. The instrument was used three times before we acquired it. The stepone realtime pcr system is a 48well, lowthroughput realtime pcr instrument perfect for both firsttime and experienced users.
Applied biosystems, a leader in technology solutions for life science research, introduces. Open the software steponeplus it can take few minutes. Field application specialist doug rains offers advices for fixing common software. Some files and registry entries are frequently left behind when you uninstall stepone software v2. Kasp chemistry for allelic discrimination performs well on an abi stepone and steponeplus machine and this stepbystep protocol will enable users to successfully setup, run and read plates on the stepone and steponeplus. Amplify sample with the steponeplus real time system qpcr.
The software constructs fourplot, sidebyside views of all data. Stepone software has not been rated by our users yet. Intended for laboratory staff responsible for the installation and maintenance of the stepone or steponeplus system 4376782 applied biosystems stepone and. This easy to use instrument comes with a builtin lcd touchscreen controllers allowing you to preconfigure experiments and save them in the system internal memory bank.